Recent publications comparing synthetic dyes for super-resolution imaging have shown CF™ dyes give the best performance for multiple methods. The superior brightness, photostability, and photochemical switching properties of certain CF™ dyes are ideal for 3-D SIM, multi-color 3-D STORM, and other super-resolution and single molecule imaging techniques.
As Lehmann and colleagues report in their recent publication in the Journal of Biophotonics, “the spectrally close dyes CF647 and CF680 comprise an optimal dye pair for spectral demixing-based, registration free multicolor dSTORM with low crosstalk. Combining this dye pair with the separately excited CF568 we performed 3-color dSTORM to image the relative nanoscale distribution of components of the endocytic machinery and the cytoskeleton.”
To learn more, click on one of the references below or download our Super-Resolution Microscopy Flyer. Find out more about CF™ dyes.
Comparison of conventional wide-field microscopy (left) with STORM (right) using CF™ dye conjugates. Fixed cells were stained with mouse anti-tubulin antibody followed by CF™ dye conjugated anti-mouse secondary antibody (top row: CF™647, middle row: CF™660C, bottom row: CF™680). See the Super-Resolution Microscopy Flyer for details of STORM imaging. Images courtesy of Sam Kenny and Professor Ke Xu, College of Chemistry, University of California, Berkeley.
CF™568 (left) produces better images than Cy®3b (right) in 3-D STORM microscopy. Fixed cells were stained with mouse anti-tubulin antibody followed by dye-conjugated anti-mouse secondary antibodies. See the Super-Resolution Microscopy Flyer for details of STORM imaging. Images courtesy of Sam Kenny and Professor Ke Xu, College of Chemistry, University of California, Berkeley.
References for CF™ Dyes in Super-Resolution Microscopy
