對于活細胞成像，Mix-n-Stain? 試劑盒可以標記更為理想的熒光化學標簽，更小，更亮，更多熒光蛋白的通用替代物。用Mix-n-Stain? 試劑盒標記不同的化學標簽可以在活細胞表面和內部得到高效率和特異性的目標蛋白。Mix-n-Stain 試劑盒同樣適用于固定細胞成像(Figure 3)和凝膠成像(詳情請下載Mix-n-Stain? Small Ligand Flyer )。
Figure 1. Live cell imaging of HeLa cells expressing CLIP-NK1R labeled with (A) CLIPamine conjugated to green fluorescent CF488A using Mix-n-Stain compared to (B) CLIP-surface 488 from New England Biolabs (NEB catalog no. S9232S). Cell nuclei were stained blue with Hoechst 33342 (catalog no. 40046).
Figure 2. Three color imaging in live HeLa cells. Cell surface protein ADRβ2 was labeled using the CF488A Mix-n-Stain kit via the SNAP-tag (green). Mitochondrial protein Cox8A was labeled using the CF540 Mix-n-Stain kit via the CLIP-tag (red). Cell nuclei were stained blue with Hoechst 33342 (cat. no. 40046).
Figure 3. Three color imaging in fixed HeLa cells. Cell surface protein NK1R was stained with CLIP-tag labeled with the CF488A Mix-n-Stain kit (green). Cells were fixed, permeabilized, stained with CF633 phalloidin (catalog no. 00046, red), and mounted with EverBrite Mounting Medium with DAPI (catalog no. 23002; nuclei: blue).
Mix-n-Stain? small ligand labeling kits for cell surface labeling are available in your choice of 10 CF? dye colors for labeling ligands for cell surface targets, and three CF? dye colors for ligands that bind intracellular targets. Also see our Mix-n-Stain? Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.
Mix-n-Stain and CF dyes are covered by US and international patents, and are trademarks of Biotium, Inc. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation; Pacific Blue, Texas Red, and Alexa Fluor are trademarks or registered trademarks of Invitrogen Corp; BD Horizon is a trademark of BD Biosciences; Cy Dye is a registered trademark of GE Healthcare; IRDye is a registered trademark of LI-COR Biosciences.
